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生理信号测量

大小鼠脑电记录仪

时间:2016-09-28来源:本站作者:玉研仪器

详细介绍

我们可以提供三通道和四通道的系统,用于研究自由活动状态下小鼠和大鼠的睡眠、癫痫、行为模式研究。脑电/肌电系统利用特色的头戴式的前置放大器,即使在动物运动时也能得到非常清晰的波形图,四通道具有所有三通道的特征,并额外增加一个通道,配置灵活,具有同时配置生物传感器的能力。

 

  • 用于实时监测动物脑电、肌电、化学物质的浓度(谷氨酸、葡萄糖、乳酸、乙醇、胆碱、总生物胺)
  • 可实现动物自由活动状态下实时监测
  • 实现信号遥测功能,动物可放于迷宫内,实时监测脑内化学物质的浓度

大小鼠脑电记录仪可以对自由活动状态下的大小鼠睡眠、癫痫和一般行为范式研究提供了一套专业的生物电记录系统。使用固定于动物头部的前置放大器(Headstage)进行信号放大,即使在动物活动时也可以持续记录到极低噪音的理想信号。

我们提供了一套预先配置好的三通道研究系统,可用于睡眠或者癫痫研究。同时还可以提供一套更加灵活的四通道系统,整合了生物探头,以便用于多种信号的同步记录。所有系统都支持光遗传学套件。常见应用:睡眠研究、癫痫研究、深部电极记录、皮层记录、场电位、认知研究

 
部分部件:
  • 头部固定器


 

预制的大小鼠头部固定器构件将连接了EEG或者EMG电极线的插件固定在微型板上。额外的两个针的电极是用于4通道EEG的配置。预制的固定器构件减少了手术时间,并且允许电极的多重排列,提供了即插即用的导线连接方式。 

  • 前置放大器


 

通过一个螺丝钉将前放固定到动物的头部。
高通滤波:0.5Hz EEG,10Hz EMG(睡眠研究);或1.0Hz EEG,10Hz EMG(癫痫)
增益:X100(睡眠),X10(癫痫)

  • 线缆系统


 

一根18英寸的线缆通过转轮链接到前置放大器。线缆中的导线外层被一层金属弹簧丝保护着。

  • 放缠绕万向轮


 

防缠绕转轮固定在动物笼顶部。转轮的两个插头设置为允许活动杆朝不同方向可以平衡的转动。

  • 数据采集和分析器

 

系统配备的一个数据调制和采集器(DCAS)提供了二级放大、滤波、数字化功能,最后经过USB线将数据传输到运行采集分析软件的计算机上。

技术参数 3通道 4通道
采样速率 200Hz-2KHz 200Hz-20Hz
低通滤波 10Hz-1KHz 20Hz-15KHz
ADC精度 14 16
输入/输出端口 1TTL I/O
3 模拟输出
4TTL I/O
4模拟输出







产品类型:
Ø 3通道:通过模拟信号输出
Ø 4通道:全配置通道,集成生物传感器,通过前置放大器的信号变换重新配置
4通道系统有着3通道系统所有的特征,除此之外还增加一个通道,配置灵活的同步生物传感器。

 


可选配件:
Ø 生物传感器
Ø 集成视频同步记录设备

 

脑化学物质实时监测模块:

快速扫描循环伏安法(FSCV)电化学检测系统,用于儿茶酚胺(i.e., dopamine, norepinephrine, and serotonin)的测量。可以用于测量自由清醒活动状态下的动物。该遥测系统适用于小鼠。

 

    FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

    Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals.

     

     

     

    HOW IT WORKS

    Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V/s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.

    l  Voltage Span: -1.1 V to +1.3 V

    l  Range: 250 – 400 V/s 

    l Sweeps/second: 5 - 10 

    l Points/sweep: 1000 

     

     

    Analysis

    Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.

     

    The TETHERED FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host PC. The system comes with Pinnacle’s FREE 8500 software.

     

     

    1. The FSCV interface box provides access to stimulus lines and transmits data to PAL-8500 software.

    2. A low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.

    3. Signals are amplified and filtered at the head of the animal using our headstage, which ensures the delivery of clean, artifact-free data.

    4. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors. Headmounts are affixed to the skull with dental acrylic and act as a connection port for the headstage.

     

     

     

     

    CARBON FIBER

     

    Pinnacle’s FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 µm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag/AgCl reference electrode.

     

    Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

     

     

     

     

    CARBON FIBER SENSORS

    Carbon fiber sensors are ordered by cannula type and whether the researcher needs to remove them from the cannula for post-calibration. CFS-F sensors are fixed in the cannula and cannot be removed for post-calibration。

     

     

    FSCV PAL-8500 SOFTWARE PACKAGE

    The FSCV system includes Pinnacle’s FREE PAL-8500 software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording, which allows monitoring of animal behavior simultaneously with biogenic amine release.

     


    Additional features of this software include:
    • Background Subtraction
    • 3D Visualization 
    • User-Selectable Filters 
    • Heat Maps 
    • Animated Voltammograms
    • Export to Third-Party Packages 

     

     

     

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