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离体组织实验

组织浴槽荧光测量系统

时间:2016-12-17来源:本站作者:玉研仪器

详细介绍

组织浴槽荧光测量系统 Fluoroplex System

 

FluoroPlex多通道荧光组织张力(心脏室压)及离子浓度同步测量灌流系统
世界上最快的多通道比率荧光组织灌流系统:FluoroPlex多通道荧光组织张力(心脏室压)及离子浓度同步测量灌流系统能提供上述一些列解决方案:
1. 多种组织适用,骨骼肌,血管,心肌、心脏,功能更强大
2. 最多可同时测量八个组织样品,大大提高实验效率
3. 单波长荧光探针采集频率高达1000HZ,双波长荧光探针采集频率高达250HZ,采集速度最快
4. 能够适用于快速离子信号变化,如:钙瞬变等指标
5. 自由更换滤光片,适应不同离子的荧光测量如Ca2+Cl-H+Na+Mg2+
6. 组织张力、心室内压与荧光的同步检测,数据的无缝连接,实时图形化数据反馈
7. 程控刺激电极,可根据实验需求自由设置不同程序,刺激电压±40V,电流24mA,场刺激时间0.4-24ms自由调节,刺激频率0.01-99HZ自由设置
8. C-Pace组件更适合长时间的细胞、组织等孵育,减少去分化,保持细胞、组织功能形态;不同设置满足不同实验要求,例如高频刺激能达到病理模型状态
9. 血管压力的多参数信息采集以满足不同实验的要求:内外径、左//平均壁厚、内//中截面面积、中膜内径比、平均压力、流速、血管壁切应力 、血管阻力、雷诺数

 

Introducing the FluoroPlex, the world’s fastest ratiometric multi-bath tissue fluorometry system.
The FluoroPlex System is a complete system that combines our Radnoti tissue bath systems with IonOptix fluorometry and AdInstruments Powerlab data acquisition. The FluoroPlex System collects quantitative fluorescence (including ratiometric data) along with force from up to eight tissue baths simultaneously. Designed with flexibility in mind, the FluoroPlex is a application-driven product that can be tailored to the specific demands of your investigation.

 

Fluorescence illumination is provided by a Fluoroplex fluorometer and stand-alone controller from IonOptix, a leading manufacture of fluorometric instruments. Fluorescence data can be collected from multiple baths at up to 1000 samplings per second. Ratiometric fluorescence can be collected at up to 250 samplings per second, ideal for quantitative calcium measurements using Fura-2. Excitation and emission optics are easily interchanged for the fluorophore of your choice. The complete FluoroPlex System includes our Radnoti tissue baths, newly designed to accommodate liquid light guides. Also included are our Radnoti force transducers, amplifiers, and thermal circulator as well as a convenient light-tight box specifically built to fit the system’s dimensions. A variety of baths and chambers are available, including 5, 10, and 25ml baths. ADInstruments PowerLab USB data acquisition interface with LabChart and Scope softwares provide data handling and analysis and work with any Mac or PC. Interfaces are available in 8- and 16-channel configurations.

 

The Fluoroplex systems are available in a 4-unit tissue bath configuration (Product Code: 180040-CS) and 8-unit tissue bath configuration (Product Code: 180080-CS).

 

Complete systems for fast quantitative fluorometry:


Burst mode

  • 250 ratios/second
  • 1000 recordings/second from single excitation dyes
  • Ideal for skeletal muscle

Continuous mode

  • 10 ratios/second from 8 baths simultaneously
  • Ideal for aortic rings and other smooth muscle preps

A key innovation of the FluoroPlex System, the FluoroPlex epifluorescence illumination and detection assembly is built for fast quantitative fluorescence photometry collected from multiple tissue baths. Tissue fluorometry is completely automated through a stand-alone controller and is capable of both single and dual excitation modes. The FluoroPlex controller features a convenient digital interface and an intuitive experiment programming dialog to optimize speed and throughput. With its intelligent controller, an ingenious light path, and fast wavelength switching, the FluoroPlex promises to provide critical new insights that advance your research.

Features

  • 75W Xenon light source: nearly uniform illumination from the UV through the visible spectrum.
  • Galvanometer-driven wavelength switching: ideal for dual excitation, the galvo enables rapid switching for quantitative ratiometric dyes such as the calcium probe Fura-2.
  • Micro-stepping multiplexer: offering precise control of light guide selection, the multiplexer steers light to as many as eight light guides.
  • Liquid light guides: providing direct interrogation of isolated tissue, liquid light guides are a highly efficient and flexible means of delivering and collecting light. Systems can be equipped with up to eight light guides to maximize experimental throughput and resources.
  • Photomultiplier tube (PMT): including an integrated high-voltage power supply and amplifier discriminator, the PMT provides reliable and sensitive light detection.
  • Innovative light path: complete with wavelength filtering specific to your fluorescent probe, the light path was painstakingly designed to minimize background while preserving signal.
  • Stand-alone controller: completely automated, intuitive interface allows easy selection of the illumination protocol. Protocols can be written in “Burst mode” for high-speed interrogation of an individual specimen before changing to the next channel, or “continuous mode” for continuous sampling of all baths. Background values are easy to collect and store. These values are automatically subtracted before data output. For dual excitation dyes, values are automatically ratioed before data output. The FluoroPlex also allows signal averaging for noise reduction.
  • High-Speed: Burst mode sampling rate is 1000 Hz (250 Hz dual excitation); Continuous mode sampling rate for four baths is 10 Hz.

Outputs

  • Digital out: the digital out sends a TTL pulse for convenient synchronization with external devices, such as an electrical field stimulator.
  • Raw channels: two raw analog outputs enable constant monitoring of raw counts.
  • Data channels: eight analog outputs deliver either background-subtracted ratiometric values for dual excitation probes or background-substracted counts for single excitation and emission dyes.

 

 

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