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离体组织实验

心脏灌流槽

时间:2016-12-17来源:本站作者:玉研仪器

详细介绍

Radnoti Heart Chambers

Radnoti Heart Chambers for Rat/Guinea Pig/Rabbit

The Radnoti Hi-Tech Heart Chamber for Rat/Guinea Pig/Rabbit has been designed for researchers using either Langendorff or working heart systems.

This chamber incorporates a number of features found in no other system. The chamber is fully water-jacketed using Radnoti Quick Disconnect fittings to permit easy removal from the rest of the system for cleaning. Both the chamber body and lid are independently mounted on support rods for easy positioning. The chamber has four ports in the chamber lid for insertion of instrumentation such as balloon catheters, electrodes, fiber-optic cables, cannula, etc. The chamber lid is secured with a threaded ring, which permits sealing the chamber for reduced heat loss and purging of the chamber interior with gas mixtures. Two of the lid access ports are placed on a locking cam for easy positioning and securing of mounting cannula, which come in a variety of sizes. The cannula are fitted into the ports using collars, which allow the cannula to be rapidly extended or retracted and then locked into place.

At the bottom of the chamber is a removable pulley which inserts into the chamber interior and is used for apical force measurements. This pulley can be easily positioned as it is secured with a collar tightened from the outside of the bath. A suture is secured to the heart and the line passed around the pulley and through the hollow pulley support arm using a wire loop provided. To close the chamber, the chamber body is slid up to the lid, the threaded ring is tightened, and then the suture is tied to the force transducer. The benefits of this system are improved temperature control, the maintenance of a perpendicular line to the transducer and the virtual elimination of perfusate aerosols, an important consideration when working with isotopes. To enhance collection of perfusate, the bottom of the chamber funnels the effluent into a tapered ground glass fitting which directs the flow out of the chamber. The Radnoti Hi-Tech Chamber is designed in three sizes: Small (rat, guinea pig); Medium (cat, rabbit); and Large (porcine) isolated heart preparations. This chamber can be retrofitted into older Radnoti heart systems. Stainless steel cannula may be ordered as an option.

Heart Chamber for Mouse Dimensions

Catalog# Chamber I.D. Chamber length Water jacket O.D. Water jacket length Store
140150 (small high tech) 49mm 100mm 70mm 110mm  
140160 (medium high tech 67mm 145mm 100mm 150mm  

Radnoti Heart Chambers for Mouse

Based on our popular Hi-Tech Heart chamber design, the Radnoti Hi-Tech Mouse Heart Chamber maintains many of it’s larger counterparts features while it’s reduced size accommodates the increasingly popular Langendorff and Working heart Mouse Heart protocols.

The chamber is fully water-jacketed using Radnoti Quick Disconnect fittings to permit easy removal from the rest of the system for cleaning. As with it’s predecessor both the chamber and the lid are independently mounted on support rods for easy positioning.While compact in size, the chamber maintains the removable pulley assembly and fiber optic ports found in the original Hi-Tech Heart Chambers.

To accommodate the mouse heart the chamber’s cannula are 20-22 gauge stainless steel at the final connection point. The body of the Cannula are large diameter to reduce resistance. Both Cannula are mounted in rotating cams to allow for ease of positioning. The Cannula vertical and horizontal position are locked via thumb screws. Two additional access ports are provided in the lid for insertion of instrumentation such as balloon catheters, electrodes, fiber optic cables, etc.

To enhance collection of perfusate, the bottom of the chamber funnels the effluent into a tapered ground glass fitting which directs the flow out of the chamber. To accommodate the mouse heart, the chamber’s cannula are 14 – 22 gauge stainless steel press fit mounted in a removable female luer to threaded hub assembly. The cannula vertical position is adjusted by threading or unthreading the hub assembly into the lid. The horizontal position of the atrial cannula can be nominally adjusted in the same manner. Two additional female luer access ports are provided in the lid for insertion of instrumentation such as balloon catheters, electrodes, fiber optic cables, etc.

 

 

Catalog# Chamber I.D. Chamber length Water jacket O.D. Water jacket length Store
130160 (mouse) 38mm 90mm 60mm 75mm  

 

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